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Preparation Method for Branch Spawn Spawn

1. **Branch & Container Prep**     - Calculate branch quantity based on daily planting bag output.     - Containers: Elongated spawn bottles, or polypropylene plastic bags (0.048-0.052mm thick; double if too thin).

 2. **Branch Soaking**     - Soak branches in limewater for ≥24 hours until no "white core" remains inside (ensures hot moist steam penetrates for full sterilization later).

 3. **Corn Paste Making & Branch Coating**     - **Corn paste ratio**: 95% water, 4.7% cornmeal/wheat flour, 0.3% agar powder.     - Cook cornmeal with most water; dissolve agar in remaining water, mix with cooked cornmeal, boil until bubbling.     - Dip soaked branches in corn paste (for nutrients/attachment) → place on table → coat with 99.5% bran + 0.5% lime mixture.

 4. **Filling the Bag**     - Line bag bottom with sawdust (prevents branch punctures) → place straight branches upright in the bag.     - Fill gaps between branches with wood culture medium (reduces trapped cold air; some add medium first, shake to fill gaps).     - Add a top layer of medium to fully cover branches ("bridging").     - Tighten a plastic collar (not too tight), insert a punch rod (for later mother spawn grafting) → seal with cotton plug (better elasticity/gas exchange than sponge), kraft paper, and rubber band.

 5. **Sterilization**     - Sterilize branch bags with growing bags (factory use: autoclave).     - Process: Heat to 100°C (hold ≥1 hour) → increase pressure (displaces cold air via air thermal expansion, lets hot moist steam penetrate).     - **Key high-pressure steps**: 105℃ (60 mins) → 118℃ (40 mins) → 125℃ (120-140 mins).     - Post-sterilization: Do a "back-grafting" test initially to check if sterilization is complete.

 6. **Success Keys**     - Branches soaked for 24 hours.     - Gaps between branches filled with sawdust medium.     - Follow the specified high-pressure sterilization process.