
1. Mother Spawn: The mycelium is pure white, dense, robust, and grows uniformly with a glossy sheen.
It is creeping, with well-developed aerial mycelium and does not produce pigment. At 22–25℃, it fully
colonizes the slant culture medium in 6–8 days. At 15℃, physiologically mature mycelium turns the test
tube slant culture medium a pale yellow color, and sometimes fruiting bodies appear.
2. Primary and Cultivation Spawn: High purity, free from contamination; pure white mycelium, uniform
and vigorous growth; moderate moisture content, adhering tightly to the bottle (or bag) wall without
shrinkage or liquid accumulation. At 22–25℃, it generally fully colonizes in 30–40 days. If the culture
medium dries out and yellow liquid accumulates at the bottom of the bottle, it is an aged spawn and
cannot be used. If yellow, green, or orange-red colors appear, it indicates contamination by Aspergillus
flavus, Phytophthora aeruginosa, or Neurospora, and the spawn must be discarded.